Molecular Biology Reagents and Kits
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt2
The pASG-IBAwt2 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via Entry Vector is possible.
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt1
The pASG-IBAwt1 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
Cayman Chemical Fungal Lysergol 250mg
An ergot alkaloid and 5-HT1A, 5-HT2A, and 5-HT2C receptor agonist (EC50s = 73, 1.6, and 6.6 nM, respectively, in a FLIPR assay); induces contractions in isolated rat tail arteries (EC50 = 436.5 nM); enhances the antibacterial activity of nalidixic acid against susceptible and drug-resistant E. coli at 10 UG/ml
Sigma Aldrich Fine Chemicals Biosciences Glyoxal solution BioReagent, for molecular biology, ~40% in H2O (~8.8 M) | 107-22-2 | MFCD00006957 | 100ML
Glyoxal solution BioReagent, for molecular biology, ~40% in H2O (~8.8 M) | Mol Wt: 58.04 | 107-22-2 | MFCD00006957 | 100ML
Bioworld URINEEXFOLIATED CELL) RNA
URINE (EXFOLIATED CELL) RNA PURIFICATION KIT: For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine. Isolate and detect total RNA from as little as 1 mL of urine; Provides high quality RNA for sensi...
Bioworld SPUTUM DNA ISOLATION KIT
A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples ar...
Bioworld LEUKOCYTE RNA PURIFICATION KIT
For the rapid extraction and purification of total RNA from leukocytes Fractionate leukocytes from whole blood in minutes; Isolate total RNA, including microRNA, without phenol; Rapid and convenient spin-column format. FEATURES AND BENE...